Isolation and characterization of Roseomonas mucosa from different clinical specimens.

Abstract

 Objective: This study is focused on isolation and identification of Rosomonas mucosa from different clinical specimens and extracted and partial purification of its pigment.

Methods:  In this study, different specimens were collected from different organs such as skin, teeth root canal, urine catheter swab and blood from Al-Yarmouk hospital and some specific clinics, through a period from October 2019 till February 2020.  The collected specimens were used to isolate the Roseomonas mucosa specifically. The bacterial colonies found in different specimens collected were isolated using blood and luria bertani media agar. Then the selected specific isolate is used for pigment extraction by ethanol. The potential pigment was characterized by ultraviolet spectroscopy, Fourier transform infrared (FT-IR) and thin layer chromatography (TLC). All these techniques were used to analyze the functional group of the extracted pigments.

Results: Primary screening shows a suspected Roseomonase sp. are 53/173 specimens. Whereas secondary screening concluded only 3 isolates showed a characteristic phenotype of Roseomonase sp. The color of the bacterial colonies is pink, mucoid and almost runny texture. The isolated colonies were examined microscopically, Roseomonas sp. was appeared as coccobacilli that form mostly pairs and short chains and identified using biochemical tests. The pigment-producing bacterial isolate was selected and propagated using suitable culture media. The extracted pigment showed the maximum spectrophotometric absorbance at 595 nm and their functional groups were identified using FT-IR analysis, containing alcohol, alkenes, alkynes phenols, alkanes, and primary amines functional groups, in addition may be included halogen compound mainly iodine. TLC result of this experiment showed that, the suspected spot that referred to an extracted pigment which migrate as brown component in the TLC sheet with RF. equal 0.87.

Conclusion: Based on the recent results, Roseomonas mucosa may adhere different surfaces because of mucosal texture, and can be isolated locally and characterized using ordinary biochemical tests, and its pigment RF. closely like R. gilardii pigment.